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Development of a DNA Photoaffinity Probe for the Analysis of 8‐OxodG‐Binding Proteins in a Human Proteome
Author(s) -
Winnacker Malte,
Welzmiller Veronika,
Strasser Ralf,
Carell Thomas
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000244
Subject(s) - photoaffinity labeling , proteome , dna , computational biology , dna repair , human proteome project , chemistry , dna mismatch repair , biochemistry , biology , binding site , proteomics , gene
Finding DNA repair factors: A comparative study with DNA probes containing 8‐oxodG and bearing different photo‐crosslinking moieties found that a triethyleneglycol‐trifluoromethyl‐phenyldiazirine system was excellent for the labeling of DNA repair factors. This probe was used for the activity‐based and selective labeling of different DNA repair factors in a human HCT‐116 nuclear proteome.