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Single‐ and Double‐Headed Chemical Probes for Detection of Active Cathepsin D in a Cancer Cell Proteome
Author(s) -
Nussbaumerová Martina,
Srp Jaroslav,
Máša Martin,
Hradilek Martin,
Šanda Miloslav,
Reiniš Milan,
Horn Martin,
Mareš Michael
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000182
Subject(s) - proteome , proteases , cathepsin , biochemistry , biotin , chemistry , computational biology , cathepsin b , molecular probe , biology , microbiology and biotechnology , enzyme , dna
Tracing aspartic proteases: We present the design of activity‐based probes for cathepsins D that are effective in a complex proteome. The probes consist of a binding module with a statin‐containing peptidic core, a warhead module bearing a photoreactive group, and a reporter tag. The fluorescent and biotin tags make the probes a powerful tool for a wide range of “in‐gel” and “on‐blot” proteomic applications.