Single‐ and Double‐Headed Chemical Probes for Detection of Active Cathepsin D in a Cancer Cell Proteome | Zendy

Nussbaumerová Martina | Zendy; Srp  Jaroslav | Zendy; Máša Martin | Zendy; Hradilek Martin | Zendy; Šanda Miloslav | Zendy; Reiniš Milan | Zendy; Horn Martin | Zendy; Mareš Michael | Zendy

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Single‐ and Double‐Headed Chemical Probes for Detection of Active Cathepsin D in a Cancer Cell Proteome

Author(s) -

Nussbaumerová Martina,

Srp Jaroslav,

Máša Martin,

Hradilek Martin,

Šanda Miloslav,

Reiniš Milan,

Horn Martin,

Mareš Michael

Publication year - 2010

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.201000182

Subject(s) - proteome , proteases , cathepsin , biochemistry , biotin , chemistry , computational biology , cathepsin b , molecular probe , biology , microbiology and biotechnology , enzyme , dna

Tracing aspartic proteases: We present the design of activity‐based probes for cathepsins D that are effective in a complex proteome. The probes consist of a binding module with a statin‐containing peptidic core, a warhead module bearing a photoreactive group, and a reporter tag. The fluorescent and biotin tags make the probes a powerful tool for a wide range of “in‐gel” and “on‐blot” proteomic applications.

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