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Artificial Metalloenzymes through Cysteine‐Selective Conjugation of Phosphines to Photoactive Yellow Protein
Author(s) -
Laan Wouter,
Muñoz Bianca K.,
den Heeten René,
Kamer Paul C. J.
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000159
Subject(s) - bioconjugation , phosphine , chemistry , cysteine , combinatorial chemistry , nucleophile , organic chemistry , catalysis , enzyme
Pinning phosphines on proteins: A method for the cysteine‐selective bioconjugation of phosphines has been developed. The photoactive yellow protein has been site‐selectively functionalized with phosphine ligands and phosphine transition metal complexes to afford artificial metalloenzymes that are active in palladium‐catalysed allylic nucleophilic substitution reactions.