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Photomodulation of Protein Trans‐Splicing Through Backbone Photocaging of the DnaE Split Intein
Author(s) -
Berrade Luis,
Kwon Youngeun,
Camarero Julio A.
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000157
Subject(s) - intein , trans splicing , protein splicing , rna splicing , chemical biology , chemistry , protein evolution , computational biology , genetics , biology , biochemistry , gene , rna
Lightly spliced inteins: A novel strategy to modulate the assembly and trans‐splicing activity of the Ssp DnaE split intein was achieved by introducing two photolabile protecting groups onto the backbone of the C‐intein polypeptide. This modification was not only able to efficiently block the trans‐splicing activity, but also reduce significantly the binding affinity constant between the C‐ and N‐intein fragments. The original activity of the wild‐type split intein could be fully recovered by brief exposure to UV light.