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Antibody Detection by Using a FRET‐Based Protein Conformational Switch
Author(s) -
Golynskiy Misha V.,
Rurup W. Frederik,
Merkx Maarten
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000143
Subject(s) - förster resonance energy transfer , antibody , computational biology , computer science , fluorescence , signal (programming language) , chemistry , biology , immunology , physics , programming language , quantum mechanics
No wash, just go: Classical antibody‐detection methods rely on heterogeneous detection schemes that involve multiple, time‐consuming binding and washing steps. Here we present a new concept to translate the antigen–antibody interaction directly into a readily detectable fluorescent signal by using a single‐chain sensor protein and taking advantage of the unique Y‐shaped structure common to all antibodies.