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Insights into Multienzyme Docking in Hybrid PKS–NRPS Megasynthetases Revealed by Heterologous Expression and Genetic Engineering
Author(s) -
Li Yanyan,
Weissman Kira J.,
Müller Rolf
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000103
Subject(s) - nonribosomal peptide , polyketide , computational biology , heterologous expression , docking (animal) , biology , heterologous , synthetic biology , mutagenesis , gene , genetics , biosynthesis , mutation , recombinant dna , medicine , nursing
A critical test: We have used Red/ET recombination in a heterologous expression vector to site‐specifically modify the pathway to the myxobacterial metabolite myxothiazol. Our mutagenesis results strengthen an existing model for interpolypeptide “docking” during biosynthesis of such hybrid polyketide–nonribosomal peptide natural products.