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Rapid Multilabel Detection of Geranylgeranylated Proteins by Using Bioorthogonal Ligation Chemistry
Author(s) -
Berry Alexandra F. H.,
Heal William P.,
Tarafder Abul K.,
Tolmachova Tanya,
Baron Rudi A.,
Seabra Miguel C.,
Tate Edward W.
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000087
Subject(s) - protein tag , bioorthogonal chemistry , computer science , information retrieval , computational biology , chemistry , biochemistry , fusion protein , recombinant dna , click chemistry , biology , combinatorial chemistry , gene
One tag, two labels : Defects in protein prenylation lead to a number of serious diseases; here we show that an azide‐tagged substrate enables multilabel detection of defective protein geranylgeranylation by two geranylgeranyl transferases. Rapid in‐gel visualization and affinity purification have been demonstrated on recombinant proteins, cell lines, and mammalian disease models.