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Perspectives on Synthetic Promoters for Biocatalysis and Biotransformation
Author(s) -
Ruth Claudia,
Glieder Anton
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900761
Subject(s) - synthetic biology , biocatalysis , promoter , computational biology , biochemical engineering , metabolic engineering , biotransformation , biology , directed evolution , gene expression , gene , microbiology and biotechnology , nanotechnology , computer science , enzyme , genetics , biochemistry , engineering , ionic liquid , materials science , mutant , catalysis
Abstract Acting on the transcriptional level, synthetic promoters have been useful tools for controlling gene expression and have applications in many fields. Here, we discuss synthetic promoters and libraries in regard to current and future applications in the field of biocatalysis or biotransformation. We also focus on synthetic promoter design principles and distinguish between prokaryotic and eukaryotic destinations. The natural toolboxes available for tuneable gene expression and the regulation of enzyme function are limited and primarily host specific. Synthetic biology offers generally applicable concepts and quick implementation. Smart alternatives to transcriptional regulation enrich the engineer's tool box for optimizing industrial enzyme production and host‐cell physiology for whole‐cell processes. Industrially applicable, tuneable enzyme cascades and artificial circuits for iterative up‐ and down‐regulation will soon be achieved.