Premium
Peptide‐Functionalized Spherical Polyelectrolyte Nanobrushes for Real‐Time Sensing of Protease Activity
Author(s) -
Yin BinCheng,
Zhang Min,
Tan Weihong,
Ye BangCe
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900735
Subject(s) - protease , homogeneous , polyelectrolyte , peptide , nanotechnology , supramolecular chemistry , combinatorial chemistry , chemistry , computer science , materials science , enzyme , physics , molecule , biochemistry , organic chemistry , polymer , thermodynamics
Magic nanobrush: We report an effective self‐assembled spherical polyelectrolyte nanobrush (SPB) composites with peptides and demonstrate its feasibility in detecting protease activity in a homogeneous solution. This assay allows rapid and accurate determination of protease kinetic parameters as well as inhibition constants. The sensitivity can be significantly improved to 1.0×10 −3 units (1.85∼5.55 n M ) thrombin. Our results demonstrate that SPBs, as a new class of nanostructures, can provide an excellent platform for enzyme studies and molecular sensing development.