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Monitoring the Activity of 2‐Oxoglutarate Dependent Histone Demethylases by NMR Spectroscopy: Direct Observation of Formaldehyde
Author(s) -
Hopkinson Richard J.,
Hamed Refaat B.,
Rose Nathan R.,
Claridge Timothy D. W.,
Schofield Christopher J.
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900713
Subject(s) - heteronuclear single quantum coherence spectroscopy , formaldehyde , demethylase , chemistry , nuclear magnetic resonance spectroscopy , demethylation , histone , biochemistry , stereochemistry , dna , gene expression , dna methylation , gene
Formaldetected: Application of 1 H‐ and 1D 13 C HSQC NMR spectroscopy methods for monitoring the activity of a 2‐oxoglutarate dependent histone N ε ‐methyllysine demethylase (JMJD2E) enabled the in situ quantitative analysis of the different components of the reaction, including the direct observation of hydrated formaldehyde resulting from enzyme‐catalysed demethylation.