A Chemical Labeling Strategy for Proteomics under Nondenaturing Conditions | Zendy

Budin  Ghyslain | Zendy; Dimala  Martin Moune | Zendy; Lamour Valérie | Zendy; Oudet Pierre | Zendy; Mioskowski Charles | Zendy; Meunier Stéphane | Zendy; Brino Laurent | Zendy; Wagner Alain | Zendy

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A Chemical Labeling Strategy for Proteomics under Nondenaturing Conditions

Author(s) -

Budin Ghyslain,

Dimala Martin Moune,

Lamour Valérie,

Oudet Pierre,

Mioskowski Charles,

Meunier Stéphane,

Brino Laurent,

Wagner Alain

Publication year - 2010

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.200900641

Subject(s) - lysis , computational biology , computer science , proteomics , chemistry , ligand (biochemistry) , combinatorial chemistry , biochemistry , biology , receptor , gene

Extra‐mild nondenaturing protocol: Fishing for unaltered protein in a lysate is a prerequisite for identifying and analyzing the architecture of a protein complex. We have shown that a noncrosslinked protein–ligand complex can resist bio‐orthogonal Staudinger ligation. The resulting target–probe–label entity is compatible with nondenaturing protein analysis.

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