Premium
A Chemical Labeling Strategy for Proteomics under Nondenaturing Conditions
Author(s) -
Budin Ghyslain,
Dimala Martin Moune,
Lamour Valérie,
Oudet Pierre,
Mioskowski Charles,
Meunier Stéphane,
Brino Laurent,
Wagner Alain
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900641
Subject(s) - lysis , computational biology , computer science , proteomics , chemistry , ligand (biochemistry) , combinatorial chemistry , biochemistry , biology , receptor , gene
Extra‐mild nondenaturing protocol: Fishing for unaltered protein in a lysate is a prerequisite for identifying and analyzing the architecture of a protein complex. We have shown that a noncrosslinked protein–ligand complex can resist bio‐orthogonal Staudinger ligation. The resulting target–probe–label entity is compatible with nondenaturing protein analysis.