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Covalent‐Bond‐Based Immobilization Approaches for Single‐Molecule Fluorescence
Author(s) -
Alemán Elvin A.,
Pedini Heidi S.,
Rueda David
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900640
Subject(s) - covalent bond , fluorescence , click chemistry , chemistry , molecule , combinatorial chemistry , avidin , monolayer , nanotechnology , nucleic acid , small molecule , biotin , organic chemistry , materials science , biochemistry , physics , quantum mechanics
Two new approaches , click‐chemistry and disulfide bond bridges, to surface‐immobilizing nucleic acids for single‐molecule fluorescence experiments using covalent bonds and self‐assembled monolayers are reported. Both approaches are specific and yield comparable results to the avidin–biotin linkage, but offer new surface‐chemical properties that might be advantageous to prevent nonspecific binding of biopolymers to the surface and to expand the range of fluorescent probes that can be employed in single‐molecule studies.