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Synthesis of Threefold Glycosylated Proteins using Click Chemistry and Genetically Encoded Unnatural Amino Acids
Author(s) -
Kaya Emine,
Gutsmiedl Katrin,
Vrabel Milan,
Müller Markus,
Thumbs Peter,
Carell Thomas
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900625
Subject(s) - click chemistry , alkene , amino acid , chemistry , alkyne , combinatorial chemistry , computer science , computational biology , biochemistry , biology , catalysis
Click to convert. Three encoded alkyne and alkene amino acids were incorporated into yellow fluorescent protein (YFP) and subsequently glycosylated by using the Cu I catalyzed click reaction, as illustrated in the scheme.