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Five‐Component Cascade Synthesis of Nucleotide Analogues in an Engineered Self‐Immobilized Enzyme Aggregate
Author(s) -
Scism Robert A.,
Bachmann Brian O.
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900620
Subject(s) - cascade , chemistry , nucleotide , component (thermodynamics) , enzyme , aggregate (composite) , synthetic biology , combinatorial chemistry , biochemistry , nanotechnology , computational biology , biology , materials science , chromatography , gene , physics , thermodynamics
Pathway in a particle: A five‐enzyme biosynthetic pathway was self‐immobilized to form a single biocatalyst for generation of purine nucleotide analogues from D ‐ribose. This method provides an alternative to engineering biosynthetic pathways in whole cells that obviates problems associated with toxicity, transport and genetic regulation.