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In Vitro Recombination Mediated by G‐Quadruplexes
Author(s) -
Boán Francisco,
GómezMárquez Jaime
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900612
Subject(s) - g quadruplex , dna , recombinant dna , molecule , in vitro , chemistry , biophysics , stereochemistry , computational biology , biology , biochemistry , gene , organic chemistry
Quartet in G major: In the annealing step of the first PCR cycles, some DNA molecules acquire the G4 structure. These G‐quadruplexes intermingle to form a bimolecular quadruplex (see figure). This intermediate structure could allow the nascent DNA to cross to another strand during extension and generate a recombinant molecule.