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Phosphopantetheinylation and Specificity of Acyl Carrier Proteins in the Mupirocin Biosynthetic Cluster
Author(s) -
Shields Jennifer A.,
Rahman Ayesha S.,
Arthur Christopher J.,
Crosby John,
Hothersall Joanne,
Simpson Thomas J.,
Thomas Christopher M.
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900565
Subject(s) - complementation , pseudomonas fluorescens , mupirocin , acyl carrier protein , polyketide synthase , polyketide , biosynthesis , biology , escherichia coli , mutant , biochemistry , plasmid , transferase , amino acid , wild type , gene , chemistry , enzyme , genetics , bacteria , staphylococcus aureus , methicillin resistant staphylococcus aureus
Acyl carrier proteins are vital for the biosynthesis of fatty acids and polyketides. The mupirocin biosynthetic cluster of Pseudomonas fluorescens encodes eleven type I ACPs embedded in its multifunctional polyketide synthase (PKS) proteins plus five predicted type II ACPs (mAcpA‐E) that are known to be essential for mupirocin biosynthesis by deletion and complementation analysis. MupN is a putative Sfp‐type phosphopantetheinyl transferase. Overexpression of three type I and three type II mupirocin ACPs in Escherichia coli , with or without mupN , followed by mass spectroscopy revealed that MupN can modify both mupirocin type I and type II ACPs to their holo‐form. The endogenous phosphopantetheinyl transferase of E. coli modified mAcpA but not mAcpC or D. Overexpression of the type II ACPs in macp deletion mutants of the mupirocin producer P. fluorescens 10586 showed that they cannot substitute for each other while hybrids between mAcpA and mAcpB indicated that, at least for mAcpB, the C‐terminal domain determines functional specificity. Amino acid alignments identified mACPs A and D as having C‐terminal extensions. Mutation of these regions generated defective ACPs, the activity of which could be restored by overexpression of the macp genes on separate plasmids.