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Chivosazoles A and F, Cytostatic Macrolides from Myxobacteria, Interfere with Actin
Author(s) -
Diestel Randi,
Irschik Herbert,
Jansen Rolf,
Khalil Mohammed W.,
Reichenbach Hans,
Sasse Florenz
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900562
Subject(s) - myxobacteria , chemistry , biology , computational biology , microbiology and biotechnology , genetics , bacteria
Chivosazoles A and F, isolated from Sorangium cellulosum , showed high antiproliferative activity with different mammalian cell lines including human cancer cells. The chivosazoles caused a delay in G2/M phase of the cell cycle, and treated cells often contained two nuclei. By labeling F‐actin it was shown that the actin cytoskeleton of the cells starts to break down after a few minutes of treatment. In vitro polymerization assays with purified G‐actin revealed that the chivosazoles inhibit actin polymerization and also cause a depolymerization of pyrene‐labeled F‐actin microfilaments prepared in vitro. Chivosazoles are new tools for the investigation of issues concerning the actin cytoskeleton and they have a different mode of action from the known microfilament‐disrupting compounds like rhizopodin and cytochalasin D.