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Preparation of Biomolecule Microstructures and Microarrays by Thiol–ene Photoimmobilization
Author(s) -
Weinrich Dirk,
Köhn Maja,
Jonkheijm Pascal,
Westerlind Ulrika,
Dehmelt Leif,
Engelkamp Hans,
Christianen Peter C. M.,
Kuhlmann Jürgen,
Maan Jan C.,
Nüsse Dirk,
Schröder Hendrik,
Wacker Ron,
Voges Edgar,
Breinbauer Rolf,
Kunz Horst,
Niemeyer Christof M.,
Waldmann Herbert
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900559
Subject(s) - biomolecule , biochip , dna microarray , protein microarray , materials science , nanotechnology , protein array analysis , chemistry , biochemistry , gene expression , gene
A mild, fast and flexible method for photoimmobilization of biomolecules based on the light‐initiated thiol–ene reaction has been developed. After investigation and optimization of various surface materials, surface chemistries and reaction parameters, microstructures and microarrays of biotin, oligonucleotides, peptides, and MUC1 tandem repeat glycopeptides were prepared with this photoimmobilization method. Furthermore, MUC1 tandem repeat glycopeptide microarrays were successfully used to probe antibodies in mouse serum obtained from vaccinated mice. Dimensions of biomolecule microstructures were shown to be freely controllable through photolithographic techniques, and features down to 5 μm in size covering an area of up to 75×25 mm were created. Use of a confocal laser microscope with a UV laser as UV‐light source enabled further reduction of biotin feature size opening access to nanostructured biochips.