Premium
Biosynthesis and Biological Screening of a Genetically Encoded Library Based on the Cyclotide MCoTI‐I
Author(s) -
Austin Jeffrey,
Wang Wan,
Puttamadappa Swamy,
Shekhtman Alexander,
Camarero Julio A.
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900534
Subject(s) - computational biology , cell sorting , synthetic biology , sorting , biosynthesis , computer science , information retrieval , biology , world wide web , gene , biochemistry , cell , programming language
Cell‐ing point : This study shows that MCoTI‐cyclotides can provide an ideal scaffold for the biosynthesis of large combinatorial libraries inside living E. coli cells. Coupled to an appropriate in vivo reporter system, this library may rapidly be screened, for example, by fluorescence‐activated cell sorting.