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Delivery of Macromolecules into Live Cells by Simple Co‐incubation with a Peptide
Author(s) -
Lee YaJung,
ErazoOliveras Alfredo,
Pellois JeanPhilippe
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900527
Subject(s) - incubation , macromolecule , peptide , chemistry , simple (philosophy) , biochemistry , philosophy , epistemology
Special delivery: Macromolecules are delivered to live cells by simple co‐incubation with the peptide E5‐TAT. The peptide induces endocytic uptake of macromolecules present in the media surrounding cells. The peptide then responds to the acidification of endocytic organelles to promote the cytosolic release of the macromolecules with which it is sequestered. A critical advantage of this approach is that the macromolecules do not need to be conjugated to the delivery peptide.