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Dehydrocoelenterazine is the Organic Substance Constituting the Prosthetic Group of Pholasin
Author(s) -
Tanaka Eiko,
Kuse Masaki,
Nishikawa Toshio
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900503
Subject(s) - dithiothreitol , photoprotein , chemistry , adduct , oxidizing agent , squid , group (periodic table) , bioluminescence , stereochemistry , organic chemistry , biochemistry , biology , ecology , enzyme
A little light analysis: Pholasin is composed of dehydrocoelenterazine (DCL), an organic substance from the photoprotein of luminous squid. Treatment of Pholasin with excess dithiothreitol (DTT) produced a solution containing a DCL–DTT adduct, whose structure was determined by LC‐MS analysis and compared with the authentic compound. This demonstrated that DCL constituted the prosthetic group of Pholasin.