Premium
C5‐Functionalized LNA: Unparalleled Hybridization Properties and Enzymatic Stability
Author(s) -
Østergaard Michael E.,
Kumar Pawan,
Baral Bharat,
Raible Daniel J.,
Santhosh Kumar T.,
Anderson Brooke A.,
Guenther Dale C.,
Deobald Lee,
Paszczynski Andrzej J.,
Sharma Pawan K.,
Hrdlicka Patrick J.
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900500
Subject(s) - nucleic acid , rna , thermal stability , locked nucleic acid , enzyme , dna , sense (electronics) , chemistry , nanotechnology , biochemistry , computational biology , combinatorial chemistry , biology , materials science , gene , organic chemistry
The best of both worlds : C5‐functionalized locked nucleic acids (LNAs) synergistically combine the beneficial features of C5‐alkynyl DNAs and conventional antisense LNA—they are straightforward to make and yet exhibit unparalleled thermal affinity toward RNA targets, extraordinary discrimination of singly mismatched RNA strands, and superb stability against degradation by 3′‐exonucleases.