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Biofunction‐Assisted Sensors Based on a New Method for Converting Aptazyme Activity into Reporter Protein Expression with High Efficiency in Wheat Germ Extract
Author(s) -
Ogawa Atsushi
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900497
Subject(s) - wheat germ , germ , biosensor , translation (biology) , messenger rna , protein expression , chemistry , microbiology and biotechnology , biochemistry , biology , computational biology , gene
Sensible wheat germ : I have developed novel biosensors based on a new method for converting aptazyme activity into easily detectable protein expression by translating aptazyme‐fused mRNA in eukaryotic wheat germ extract. Some newly elucidated unique features of the wheat germ translation system make the sensitivity of this method much higher than that of previously reported aptazyme‐based biosensors.