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Combining Mass Spectrometry and Peptide Arrays to Profile the Specificities of Histone Deacetylases
Author(s) -
GurardLevin Zachary A.,
Kim Joohoon,
Mrksich Milan
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900417
Subject(s) - histone , peptide , histone deacetylase , enzyme , computational biology , biochemistry , chemistry , hdac10 , substrate (aquarium) , mass spectrometry , information retrieval , computer science , biology , chromatography , gene , ecology
In profile : We describe the use of peptide arrays to probe the substrate specificities of several histone deacetylase (HDAC) enzymes. The combination with SAMDI‐MS avoids the need for labels, which we demonstrate can perturb enzyme activity and lead to altered enzyme specificity.