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Regulation of Thrombin Activity with a Bifunctional Aptamer and Hemin: Development of a New Anticoagulant and Antidote Pair
Author(s) -
Wang Jing,
Cao Ya,
Chen Guifang,
Li Genxi
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900408
Subject(s) - hemin , antidote , aptamer , thrombin , bifunctional , chemistry , coagulation , biochemistry , thrombin generation , combinatorial chemistry , enzyme , biology , platelet , medicine , microbiology and biotechnology , organic chemistry , immunology , heme , toxicity , catalysis
Through thick and thin : A bifunctional aptamer (BA) and hemin have been designed as an anticoagulant and antidote pair. BA, as anticoagulant, inhibits thrombin by forming a BA–thrombin complex. Because of the higher affinity between hemin and BA, once hemin is added, it replaces thrombin to form a BA–hemin complex. Thrombin is then released and reactivated to exhibit blood‐clotting activity.