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Introduction of an Aliphatic Ketone into Recombinant Proteins in a Bacterial Strain that Overexpresses an Editing‐Impaired Leucyl‐tRNA Synthetase
Author(s) -
Tang Yi,
Wang Pin,
Van Deventer James A.,
Link A. James,
Tirrell David A.
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900407
Subject(s) - ketone , transfer rna , leucine , biochemistry , amino acid , recombinant dna , chemistry , amino acyl trna synthetases , escherichia coli , stereochemistry , rna , organic chemistry , gene
Like leucine? A leucine analogue containing a ketone has been incorporated into proteins in E. coli. Only E. coli strains overexpressing an editing‐deficient leucyl‐tRNA synthetase were capable of synthesizing proteins with the aliphatic ketone amino acid. Modification of ketone‐containing proteins under mild conditions has been demonstrated.