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SSB‐Assisted Duplex Invasion of Preorganized PNA into Double‐Stranded DNA
Author(s) -
Ishizuka Takumi,
Tedeschi Tullia,
Corradini Roberto,
Komiyama Makoto,
Sforza Stefano,
Marchelli Rosangela
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900381
Subject(s) - duplex (building) , circular dichroism , dna , single strand , stereochemistry , chemistry , computational biology , biophysics , biology , crystallography , microbiology and biotechnology , genetics , biochemistry
Invasion of the DNA snatchers : We show that one strand of decamer D K α ‐PNA efficiently invades into double‐stranded DNA (dsDNA) by utilizing the SSB‐assisted duplex invasion. An extensive analysis of D K α ‐PNA–DNA duplex formation performed by calculation of the thermodynamic parameters and circular dichroism experiments ascertained that a right‐handed helical preorganization is a very important contribution when one PNA strand invades into dsDNA.