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In Situ Monitoring of Backbone Thioester Exchange by 19 F NMR
Author(s) -
Pomerantz William C.,
Hadley Erik B.,
Fry Charles G.,
Gellman Samuel H.
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900380
Subject(s) - thioester , antiparallel (mathematics) , chemistry , coiled coil , in situ , analytical chemistry (journal) , chromatography , organic chemistry , physics , biochemistry , quantum mechanics , magnetic field , enzyme
19 F NMR to monitor BTE : A new strategy for assessing polypeptide tertiary structural stability by using thiol–thioester exchange is described. The backbone thioester exchange (BTE) method has previously been used to evaluate fold stability with HPLC measurements. Here we show that BTE can be followed by 19 F NMR, which offers technical advantages. This technique was used for rapid identification of the most stable antiparallel coiled coil in a small library of new sequences.