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Label‐Free Colorimetric Screening of Nuclease Activity and Substrates by Using Unmodified Gold Nanoparticles
Author(s) -
Lou Xinhui,
Xiao Yi,
Wang Yi,
Mao Hongju,
Zhao Jianlong
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900355
Subject(s) - nuclease , colloidal gold , substrate (aquarium) , chemistry , nanoparticle , combinatorial chemistry , nanotechnology , computer science , biochemistry , computational biology , enzyme , biology , materials science , ecology
Don't get the blues : The dNMPs produced by nucleases can better stabilize unmodified AuNPs than ss‐ or dsDNA with same base composition, thus the AuNPs remain red in contrast to the blue‐purple change observed in the absence of nucleases or substrates. This color change can be used to monitor the activity of the enzyme and allows for label‐free substrate screening.