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Specific or Nonspecific Protein–Polyphenol Interactions? Discrimination in Real Time by Surface Plasmon Resonance
Author(s) -
DouatCasassus Céline,
Chassaing Stefan,
Di Primo Carmelo,
Quideau Stéphane
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900287
Subject(s) - surface plasmon resonance , polyphenol , chemistry , nanotechnology , resonance (particle physics) , combinatorial chemistry , biophysics , biochemistry , materials science , biology , physics , nanoparticle , particle physics , antioxidant
Searching for polyphenolic keys : Immobilization of the polyphenolic molecule (−)‐vescalin onto a carboxylated dextran‐coated gold surface enabled the determination by surface plasmon resonance (SPR) of its strong (subnanomolar) affinity to the human DNA topoisomerase IIα protein target without perturbations from nonspecific complexation phenomena.