Premium
A Genetically Encoded Direct Sensor of Antibody–Antigen Interactions
Author(s) -
Mills Jeremy H.,
Lee Hyun Soo,
Liu Chang C.,
Wang Jiangyun,
Schultz Peter G.
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900254
Subject(s) - antigen , antibody , computational biology , genetically modified organism , chemistry , biology , microbiology and biotechnology , genetics , gene
Fluorescent FABS : We have genetically encoded a 7‐hydroxycoumarin moiety in an antibody combining site. The fluorescent Fab fragment reports change in fluorescence intensity upon binding of its antigen.