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Bipartite Tetracysteine Display Requires Site Flexibility for ReAsH Coordination
Author(s) -
Goodman Jessica L.,
Fried Daniel B.,
Schepartz Alanna
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900207
Subject(s) - flexibility (engineering) , bipartite graph , intramolecular force , binding site , chemistry , nanotechnology , computer science , stereochemistry , materials science , biochemistry , theoretical computer science , mathematics , graph , statistics
Flexibility required : We designed intramolecular bipartite tetracysteine sites in loops of p53 and the β‐sheets of EmGFP. We found that ReAsH binding preferentially favors tetracysteine sites with flexible geometries such as loops; flexibility was assessed by comparing Cα B‐factor values. This information is important for directing successful bipartite tetracysteine site designs.

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