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Selective Antifolates for Chemically Labeling Proteins in Mammalian Cells
Author(s) -
Pedró Rosa Laura E.,
Reddy D. Rajasekhar,
Queener Sherry F.,
Miller Lawrence W.
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900152
Subject(s) - dihydrofolate reductase , chemical biology , antifolate , biochemistry , chemistry , fusion protein , structural biology , computational biology , methotrexate , biology , enzyme , recombinant dna , antimetabolite , gene , immunology
Antifolate labels : Molecules that bind specifically and with high affinity to proteins can be developed into powerful tools for chemical biology. The interaction between substituted 5‐benzyl pyrimidines and dihydrofolate reductase can be exploited for chemically labeling fusion proteins in mammalian cells.