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Steric Constraints Dependent on Nucleobase Pair Orientation Vary in Different DNA Polymerase Active Sites
Author(s) -
Streckenbach Frank,
Rangam Gopinath,
Möller Heiko M.,
Marx Andreas
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900123
Subject(s) - steric effects , nucleobase , dna polymerase , dna , selectivity , polymerase , chemistry , substrate (aquarium) , stereochemistry , biology , biochemistry , catalysis , ecology
Abstract Finding the right fit : Herein, we report on the development of novel steric probes and present initial insights into their interplay with DNA polymerases. Our findings provide experimental evidence for varied enzyme–substrate interactions that might account for the varied selectivity previously observed.