Premium
Rolling‐Circle Amplification: Unshared Advantages in miRNA Detection
Author(s) -
Neubacher Saskia,
Arenz Christoph
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900116
Subject(s) - rolling circle replication , microrna , computational biology , rna ligase , biology , rna , nucleotide , genetics , microbiology and biotechnology , dna , gene , polymerase
Roll with it : The quantitative analysis of specific miRNAs from biological samples is very likely to revolutionize diagnostics of human disease. A novel method for miRNA analysis employing rolling‐circle amplification (RCA) can homogeneously detect miRNA, even at concentrations as low as 10 f M . The use of T4 RNA ligase 2 (T4 RnL2) at elevated temperatures enables very good discrimination of miRNAs differing by a single nucleotide.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom