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Photocrosslinking of RNA and PhotoMet‐Containing Amphiphilic α‐Helical Peptides
Author(s) -
Hyun Soonsil,
Han Areum,
Yu Jaehoon
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900100
Subject(s) - covalent bond , amphiphile , rna , adduct , peptide , chemistry , combinatorial chemistry , stereochemistry , biochemistry , biophysics , biology , organic chemistry , polymer , copolymer , gene
Discovering RNA–protein interactions : A library of photoMet‐containing peptides was synthesized by using an Arg‐ and Leu‐rich α‐helical amphiphilic peptide. Irradiation of mixtures of these peptides and Rev‐responsive element (RRE) hairpin RNA promoted formation of covalent adducts. Analysis of one adduct showed that U26 in the bulged stem is responsible for covalent bond formation with the carbene intermediate. This strategy can provide important structural information about RNA–peptide interactions.