Premium
Photochemical Regulation of Restriction Endonuclease Activity
Author(s) -
Young Douglas D.,
Govan Jeane M.,
Lively Mark O.,
Deiters Alexander
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900090
Subject(s) - endonuclease , restriction enzyme , dna , cleavage (geology) , enzyme , chemistry , biophysics , substrate specificity , biochemistry , dna damage , biology , paleontology , fracture (geology)
Removal by the light : The photochemical regulation of restriction endonucleases, which are important enzymes in molecular biology, has been investigated. Photolabile protecting groups have been installed on DNA substrates and have been demonstrated to inhibit restriction endonuclease activity until removed by UV light irradiation. Interestingly, these groups do not appear to dramatically affect initial binding of the enzyme to the DNA substrate, but rather prevent recognition of the specific cleavage site.