Functional Analysis of MycE and MycF, Two  O ‐Methyltransferases Involved in the Biosynthesis of Mycinamicin Macrolide Antibiotics | Zendy

Li Shengying | Zendy; Anzai Yojiro | Zendy; Kinoshita Kenji | Zendy; Kato Fumio | Zendy; Sherman David H. | Zendy

Premium

Functional Analysis of MycE and MycF, Two O ‐Methyltransferases Involved in the Biosynthesis of Mycinamicin Macrolide Antibiotics

Author(s) -

Li Shengying,

Anzai Yojiro,

Kinoshita Kenji,

Kato Fumio,

Sherman David H.

Publication year - 2009

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.200900088

Subject(s) - methyltransferase , biosynthesis , polyketide synthase , polyketide , enzyme , transferase , antibiotics , biochemistry , o methyltransferase , in vitro , chemistry , methionine , stereochemistry , biology , methylation , gene , amino acid

Mg motors : We characterized the in vitro function of MycE and MycF, two O ‐methyltransferases involved in the biosynthesis of mycinamicin antibiotics. Each enzyme was confirmed to be an S ‐adenosyl‐ L ‐methionine (SAM)‐dependent deoxysugar methyltransferase. Their optimal activities require the presence of Mg 2+ . With the reconstituted in vitro assays, the order of mycinamicin VI→III→IV in the post‐PKS (polyketide synthase) tailoring pathway of mycinamicin was unambiguously determined.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research