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Functional Analysis of MycE and MycF, Two O ‐Methyltransferases Involved in the Biosynthesis of Mycinamicin Macrolide Antibiotics
Author(s) -
Li Shengying,
Anzai Yojiro,
Kinoshita Kenji,
Kato Fumio,
Sherman David H.
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900088
Subject(s) - methyltransferase , biosynthesis , polyketide synthase , polyketide , enzyme , transferase , antibiotics , biochemistry , o methyltransferase , in vitro , chemistry , methionine , stereochemistry , biology , methylation , gene , amino acid
Mg motors : We characterized the in vitro function of MycE and MycF, two O ‐methyltransferases involved in the biosynthesis of mycinamicin antibiotics. Each enzyme was confirmed to be an S ‐adenosyl‐ L ‐methionine (SAM)‐dependent deoxysugar methyltransferase. Their optimal activities require the presence of Mg 2+ . With the reconstituted in vitro assays, the order of mycinamicin VI→III→IV in the post‐PKS (polyketide synthase) tailoring pathway of mycinamicin was unambiguously determined.