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Engineered Two‐Helix Small Proteins for Molecular Recognition
Author(s) -
Webster Jack M.,
Zhang Rong,
Gambhir Sanjiv S.,
Cheng Zhen,
Syud Faisal A.
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200900062
Subject(s) - helix (gastropod) , protein engineering , small molecule , chemistry , affinities , rational design , molecular recognition , protein design , computational biology , triple helix , protein structure , molecule , stereochemistry , biochemistry , nanotechnology , biology , materials science , enzyme , ecology , organic chemistry , snail
Abstract Less is more : By starting with a high‐affinity HER2‐binding 3‐helix affibody molecule, we successfully developed 2‐helix small protein binders with 5 n M affinities by using a combination of several different strategies. Our efforts clearly suggest that 2‐helix small proteins against important tumor targets can be obtained by rational protein design and engineering.