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Differential Regulation of Protein Subdomain Activity with Caged Bivalent Ligands
Author(s) -
Mayer Günter,
Müller Jens,
Mack Timo,
Freitag Daniel F.,
Höver Thomas,
Pötzsch Bernd,
Heckel Alexander
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800814
Subject(s) - aptamer , bivalent (engine) , chemistry , biophysics , computational biology , biology , genetics , organic chemistry , metal
Subtle change : Spatiotemporal modulation of individual protein subdomains with light as the trigger signal becomes possible by using bivalent aptamers and introducing photolabile “caging groups” to switch individual aptamer modules ON or OFF differentially. To the best of our knowledge, this is the first study to show that it is possible to modulate individual domain activity in aptamers, and thus also domain activity in proteins, with light.