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Synthesis of a Fluorogenic Analogue of Sphingosine‐1‐Phosphate and Its Use to Determine Sphingosine‐1‐Phosphate Lyase Activity
Author(s) -
Bedia Carmen,
Camacho Luz,
Casas Josefina,
Abad José Luis,
Delgado Antonio,
Van Veldhoven Paul P.,
Fabriàs Gemma
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800809
Subject(s) - sphingosine , lyase , sphingosine 1 phosphate , enzyme , phosphate , biochemistry , chemistry , atp citrate lyase , receptor , citrate synthase
Abstract Illuminating an ER enzyme : We report on the design and synthesis of a fluorogenic chemical sensor ( 1 ) to measure sphingosine‐1‐phosphate lyase activity in high‐throughput screening formats, as well as its validation using lyase knockout ( Sgpl1−/− ) cells.