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Formylglycine Aldehyde Tag—Protein Engineering through a Novel Post‐translational Modification
Author(s) -
Frese MarcAndré,
Dierks Thomas
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800801
Subject(s) - posttranslational modification , aldehyde , cysteine , chemistry , biochemistry , protein engineering , residue (chemistry) , computational biology , combinatorial chemistry , biology , enzyme , catalysis
Oxidation of a specific cysteine residue to Cα‐formylglycine is a novel post‐translational modification that is directed by a short recognition motif commonly found in pro‐ and eukaryotic sulfatases. As recently shown by C. Bertozzi and co‐workers, this system can be employed in protein engineering to equip proteins with genetically encoded aldehyde tags for site‐specific labeling, conjugation and immobilization.