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Protein Recognition of Platinated DNA
Author(s) -
Sadler Peter J.
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800733
Subject(s) - dna , benzophenone , oligonucleotide , chemistry , biochemistry , cisplatin , biology , genetics , photochemistry , chemotherapy
A cisplatin analogue with one of the NH 3 ligands replaced by a photoactivatable benzophenone has been used by Lippard et al. to elucidate which proteins recognise platinated DNA. Platinated oligonucleotides exposed to cancer cell extracts were irradiated to induce protein–DNA crosslinks. The benzophenone fragment gives rise to a diradical that attacks nearby protein. The proteins thus identified include some that preferentially recognise 1,2‐ or 1.3‐crosslinks.