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Sortase‐Mediated Ligation: A Gift from Gram‐Positive Bacteria to Protein Engineering
Author(s) -
Tsukiji Shinya,
Nagamune Teruyuki
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800724
Subject(s) - sortase , sortase a , ligation , protein engineering , bacteria , biochemistry , chemistry , bacterial protein , chemical ligation , enzyme , biology , peptide , microbiology and biotechnology , genetics , gene
A new enzymatic protein ligation tool , sortase, has recently emerged from Gram‐positive bacteria. This article outlines the technique, sortase‐mediated ligation, and its applications in protein engineering, which include the introduction of unnatural molecules into proteins, protein immobilization, protein–protein conjugation, protein cyclization, as a self‐cleavable tag for protein expression, protein–PNA hybrids, neoglycoconjugates, and cell‐surface protein labeling, etc.