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Dispersed Phantom Scatterer Technique Reveals Subtle Differences in Substrate Recognition by Phospholipase D Inactive Mutants
Author(s) -
Morasso Carlo,
Bellini Tommaso,
Monti Daniela,
Bassi Mattia,
Prosperi Davide,
Riva Sergio
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800718
Subject(s) - lysophosphatidylcholine , mutant , substrate (aquarium) , imaging phantom , biophysics , chemistry , materials science , biochemistry , nanotechnology , biology , physics , optics , phosphatidylcholine , phospholipid , gene , ecology , membrane
Stretch out : An elastic light‐scattering‐based method that makes use of phantom nanoparticles as a substrate organizer (see illustration) allowed the quantitative evaluation of the molecular recognition events between a series of inactivated mutants of phospholipase D and lysophosphatidylcholine. The results highlight the remarkable effects on binding capability caused by single amino acid substitutions.