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Gene Silencing in Mammalian Cells with Light‐Activated Antisense Agents
Author(s) -
Young Douglas D.,
Lusic Hrvoje,
Lively Mark O.,
Yoder Jeffrey A.,
Deiters Alexander
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800627
Subject(s) - gene silencing , chemistry , gene , sense (electronics) , microbiology and biotechnology , blue light , biology , biophysics , biochemistry , materials science , optoelectronics
On and off the spot : The spatio‐temporal light‐activation of gene silencing was achieved through the complete inactivation of phosphorothioate antisense agents by installing light‐removable (caged) groups at specific sites, as illustrated here. Full antisense activity was restored through a brief irradiation with UV light at 365 nm.