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Segmental Isotopic Labelling of a Multidomain Protein by Protein Ligation by Protein Trans‐Splicing
Author(s) -
Muona Mikko,
Aranko A. Sesilja,
Iwai Hideo
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800604
Subject(s) - labelling , rna splicing , computational biology , protein splicing , isotopic labeling , protein function , function (biology) , computer science , biology , chemistry , biochemistry , genetics , gene , rna , organic chemistry
Structure–function relationships of intact multidomain proteins : Segmental isotopic labelling of multidomain proteins could open a new avenue for studying particular domains in intact proteins, because it could simplify the NMR spectra through the incorporation of stable isotopes in a region‐specific manner. We have demonstrated that the necessary protein ligation can be easily achieved both in vivo and in vitro by protein trans‐splicing.