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The High Resolution NMR Structure of Parvulustat (Z‐2685) from Streptomyces parvulus FH‐1641: Comparison with Tendamistat from Streptomyces tendae 4158
Author(s) -
Rehm Stephan,
Han Sigeng,
Hassani Ismail,
Sokocevic Alma,
Jonker Hendrik R. A.,
Engels Joachim W.,
Schwalbe Harald
Publication year - 2009
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800547
Subject(s) - heteronuclear molecule , streptomyces , stereochemistry , active site , protein secondary structure , chemistry , proton nmr , crystallography , nuclear magnetic resonance spectroscopy , biochemistry , enzyme , biology , bacteria , genetics
The protein parvulustat (Z‐2685) from Streptomyces parvulus comprises 78 amino acids and functions as a highly efficient α‐amylase inhibitor. Parvulustat shares 29.6 % overall amino acid sequence identity to the well‐known α ‐amylase inhibitor tendamistat. Among the conserved residues are the two disulfide bridges (C9–C25, C43–C70) and the active‐site motif (W16, R17, Y18). Here, we report the high‐resolution NMR structure of parvulustat based on NOEs, J couplings, chemical shifts and hydrogen‐exchange data. In addition, we studied the dynamical properties of parvulustat by heteronuclear relaxation measurements. We compare the structure of parvulustat with the structure of tendamistat in terms of secondary structure elements, charges and hydrophobicity. The overall structural composition is very similar, but there are distinct differences including the active‐site region. These structural and dynamical differences indicate that for parvulustat an induced‐fit mechanism for binding to α ‐amylase might take place, since the structure of tendamistat does not change upon binding to α ‐amylase.