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Detection of Ligand Binding to Nucleotide Sugar Transporters by STD NMR Spectroscopy
Author(s) -
Maggioni Andrea,
von Itzstein Mark,
Tiralongo Joe,
Haselhorst Thomas
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800526
Subject(s) - golgi apparatus , biochemistry , transmembrane protein , golgi membrane , nucleotide sugar , nuclear magnetic resonance spectroscopy , function (biology) , transporter , organelle , sialic acid , atp binding cassette transporter , ligand (biochemistry) , nucleotide , biology , chemistry , sugar , computational biology , microbiology and biotechnology , stereochemistry , gene , endoplasmic reticulum , receptor
An isolated cellular organelle (Golgi‐enriched fraction) that incorporates transmembrane nucleotide sugar transporters for ligand‐binding studies has been direct interrogated by STD NMR spectroscopy. Our results show that this approach can be used for the analysis of both the overexpressed Golgi‐resident CMP‐sialic acid transport protein and endogenous NSTs. Importantly, the use of a Golgi‐enriched fraction means that the contribution of the membrane environment in protein function is not ignored; in fact it is a fundamental component of the analysis.