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Importance of Translation–Replication Balance for Efficient Replication by the Self‐Encoded Replicase
Author(s) -
Ichihashi Norikazu,
Matsuura Tomoaki,
Kita Hiroshi,
Hosoda Kazufumi,
Sunami Takeshi,
Tsukada Koji,
Yomo Tetsuya
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800518
Subject(s) - replication (statistics) , translation (biology) , balance (ability) , rna dependent rna polymerase , biology , computational biology , genetics , virology , rna , neuroscience , gene , messenger rna
In all living systems, the genetic information is replicated by the self‐encoded replicase (Rep); this can be said to be a self‐encoding system. Recently, we constructed a self‐encoding system in liposomes as an artificial cell model, consisting of a reconstituted translation system and an RNA encoding the catalytic subunit of Qβ Rep and the RNA was replicated by the self‐encoded Rep produced by the translation reaction. In this system, both the ribosome (Rib) and Rep bind to the same RNA for translation and replication, respectively. Thus, there could be a dilemma: effective RNA replication requires high levels of Rep translation, but excessive translation in turn inhibits replication. Herein, we actually observed the competition between the Rib and Rep, and evaluated the effect for RNA replication by constructing a kinetic model that quantitatively explained the behavior of the self‐encoding system. Both the experimental and theoretical results consistently indicated that the balance between translation and replication is critical for an efficient self‐encoded system, and we determined the optimum balance.